Preparative Chromatography

The loading of preparative columns is considered both practically and theoretically including the maximum column loading capacity, the maximum sample volume, volume overload, and mass overload. Preparative chromatography apparatus is then described including, solvent reservoirs, pumps, sample valves and the preparative columns themselves. The special requirements of preparative column detectors are also discussed together with the use of fraction collectors. The special packing techniques necessary for both preparative gas chromatography and liquid chromatography columns are described including both the radial and axial compression techniques. Procedures such as recycling, together with the moving bed and simulated moving bed systems are discussed in detail and some examples of the use of the moving bed system included.

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